If you leave any missing values, that row will simply be ignored. If you are not ready to enter your own data, choose sample data and choose: t test - Paired.Įnter the data for each group into a separate column, with matched values on the same row. This includes the mean and standard deviation (this information can be found in the ‘ Descriptive statistics’ sheet) for each group, as well as the t statistic, degrees of freedom and the all-important p value.From the Welcome (or New Table and graph) dialog, choose the Column tab. “There was a higher number of cells before (10257 ± 1430 cells) than after (5239 ± 861 cells) a 6-hour treatment with drug X, t(6) = 13.55, p < 0.0001.” If we were to report the results of this paired t test in a single sentence, we could write: In which case, we reject the null hypothesis and accept the alternative hypothesis. So, after performing the paired T-test in GraphPad we now know that there is a significant difference between the number of cells before and after the treatment with drug X for 6-hours. In this case a p value of < 0.0001 indicates a very significant difference, since this is less that our level of significance threshold of 0.05. The exact p value is given next to ‘ P value’. Interpretationīy looking at the ‘ Significantly different? (P<0.05)’ output, a ‘ Yes’ is given which essentially means that our results are significantly different from each other. GraphPad also gives you other descriptive information for each dataset (under the ‘ How big is the difference?’ section) and an indication for how effective the pairing between the datasets was (under the ‘ How effective was the pairing?’ section).
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